Test instructions – Type 7

These instructions (type 7) are for those test kits:

  • Code FOB – Faecal occult blood test kit (bowel cancer) for a stool sample

Test instructions:

  • Before starting the test, read these instructions through carefully.
  • Remove the film from the test device just before you carry out the test.
  • Special notes:
    • Do not take a sample if the patient has haemorrhoids which are bleeding, or during menstruation.
    • If the test subject has eaten meat, offal or food containing iron, the results of the chemical test method may show positive.
  • Components of the test kit:
    • Test cassette
    • Bottle with buffer solution and test stick
  • Carrying out the test
    1. Unscrew the bottle of buffer solution (violet lid) and take out the test stick.
    2. Take the sample by inserting the test stick into the faecal specimen in three different places.
    3. Replace the test stick in the bottle and screw the lid shut.
    4. Shake the buffer solution bottle until the sample has dissolved.
    5. Open the bottle (transparent lid) and add 3 drops of the solution through the aperture marked ‘S’ of the test cassette.
  1. Reading off the result:
    In general the lines do not have to be exactly the same, one line can be a little lighter in colour than the other. In addition traces of blood may sometimes be visible in the test device, but they have no further effect on the result.

    Read the chemical method result by using the sample pad (orange window, marked ‘Chem’) after 1 minute  and the immune colloidal method result after 5 minutes through the large result window (marked ‘C T’).

    Interpretation of chemical method:
    a) Positive: within 1 minute of adding the sample, the colour of the sample pad changes, from orange to yellowish-green or dark green.
    b) Negative: within 1 minute of adding the sample, the colour of the sample pad does not change.
    c) Invalid: The sample pad changes colour to a different colour. You should repeat the procedure using a new test, following these instructions closely.

    Interpretation of the immune colloidal method:
    a) Positive: in addition to the control stripe ‘C’, a distinct coloured stripe appears in test area ‘T’.
    b) Negative: there is only a coloured stripe in control area ‘C’. There is no stripe visible in test area ‘T’.
    c) Invalid: If no stripe is visible either in the test area or the control area, or there is only a stripe in the test area, the test is invalid. You should repeat the procedure using a new test, following these instructions closely.

    Definitive interpretation:
    a) Both methods show positive results: the final result is positive, which indicates blood in the specimen.
    >>> Please consult a doctor immediately and have the results compared with a laboratory test.
    b) The result of the immune colloidal method is positive, and that of the chemical method is negative: this indicates concealed bleeding; the concentration of haemoglobin in the specimen is much lower. The result is therefore positive.
    >>> Please consult a doctor immediately and have the results compared with a laboratory test
    c) The result of the immune colloidal method is negative, but that of the chemical method is positive: this indicates a high haemoglobin concentration which triggers an antigen excess and has a distorting effect. The specimen should be diluted 100-fold and tested again.
    c) i) If the immune colloidal method result then comes out positive, this indicates severe internal bleeding. Please consult a doctor immediately and have the results compared with a laboratory test.
    c) ii) If the immune colloidal method result remains negative, the result has been distorted by interfering substances, and the result can be regarded as being negative.
    d) Both methods present negative results: the final result is negative. If symptoms persist, please carry out another test or consult a doctor.